DNA

Part:BBa_K4663025

Designed by: Lorenzo da Silva Saraiva   Group: iGEM23_WageningenUR   (2023-10-12)


SuCas12a2

Type V Cas nuclease triggering an abortive infection phenotype (non-specifically cleavind ss-, ds.- DNA and ss-RNA) when it recognizes RNA complementary to crRNA.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 646
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 1810
    Illegal EcoRI site found at 2281
    Illegal EcoRI site found at 2299
    Illegal EcoRI site found at 2479
    Illegal EcoRI site found at 2623
    Illegal PstI site found at 2125
    Illegal PstI site found at 3513
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 646
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 1810
    Illegal EcoRI site found at 2281
    Illegal EcoRI site found at 2299
    Illegal EcoRI site found at 2479
    Illegal EcoRI site found at 2623
    Illegal PstI site found at 2125
    Illegal PstI site found at 3513
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 646
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 1810
    Illegal EcoRI site found at 2281
    Illegal EcoRI site found at 2299
    Illegal EcoRI site found at 2479
    Illegal EcoRI site found at 2623
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 646
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 1810
    Illegal EcoRI site found at 2281
    Illegal EcoRI site found at 2299
    Illegal EcoRI site found at 2479
    Illegal EcoRI site found at 2623
    Illegal PstI site found at 2125
    Illegal PstI site found at 3513
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 646
    Illegal EcoRI site found at 1342
    Illegal EcoRI site found at 1810
    Illegal EcoRI site found at 2281
    Illegal EcoRI site found at 2299
    Illegal EcoRI site found at 2479
    Illegal EcoRI site found at 2623
    Illegal PstI site found at 2125
    Illegal PstI site found at 3513
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1239
    Illegal BsaI.rc site found at 3436


[edit]
Categories
Parameters
None